Hi all,
Thanks to those who replied to my question. I thought others
might be interested, so I'm giving the summary below.
I think, we'll go for the Superdex, as we have more experience with it.
1) If the prices are similar then toyopearl may be a better choice as it has
slightly better mechanical stability and slightly higher resolution.
However, if memory serves Superdex used to be considerably cheaper.
Artem
2) In my experience (and probably yours too) what works well for
one protein doesn't necessarily work well for another. We certainly
notice that for some proteins our fancy Superdex-200 performs worse
than the much cheaper Sephacryl-200 column. I guess that the same
will hold for the Toyopearl resin. This doesn't really help you in
taking the decision but if you can afford, I wouldn't exlude one for the other.
Regards,
Boaz
3) Toyo does not have anything comparable to Superdex 10 micron beads.
For preparative scale, Toyopearl S-grade is nominally comparable to
Superdex prep grade but Superedex still has lower size dispersion
(hence better resolution) and wider dynamic resolution range.
I'd stick to Superdex for versatility unless the aim is to have a
dynamic range in the very high MW (HW-75) or to save money when
buying bulk beads.
Dima
At 04:48 PM 2/23/2007, you wrote:
Dear folks,
I have a question related to protein purification:
We are thinking to buy a new GF column. We already have the
HiLoad-superdex-75; we want to get the superdex-200 pg.
However, some colleague mentioned the Toyopearl-HW as another
alternative for Gel filteration.
I wonder if people could share their experience with Toyopearl
vs. Superdex GF columns.
regards,
Ibrahim
Ibrahim M.Moustafa, Ph.D.
Pennsylvania State University
Biochemistry & Molecular Biology Dept.
201 Althouse Lab.
University Park, PA16802
Tel (814) 863 8703
Fax (814) 865 7927
Ibrahim M.Moustafa, Ph.D.
Pennsylvania State University
Biochemistry & Molecular Biology Dept.
201 Althouse Lab.
University Park, PA16802
Tel (814) 863 8703
Fax (814) 865 7927
