Hey all, this is wildly off-topic, but since we were even talking about NMR the other day, I was thinking why not EM?
I'm trying to get negative stain images of a membrane protein. Problem is that the detergent (similar to triton) that best stabilizes the protein eats the carbon grid, even at concentrations of 0.1x CMC. Has anyone encountered a similar problem and found a solution around it? Are there different types of carbon grids? Thanks. Andreas
