Hi,
I would suggest crystallizing zinc free bovine insulin. It is cheap
and once you remove the zinc, the protein easily crystallizes in
Space Group I2(1)3 with a = 78Å.
If you then collect a 180 degree or more data set using Cu K alpha X-
rays you should easily find the three disulfides and produce an
interpretable sulfur SAS electron density map.
We use it here for teaching all the time.
Regards,
John
John Rose Ph.D.
Associate Professor
B204B, The Fred C. Davison Life Sciences Complex
120 Green Street
Department of Biochemistry and Molecular Biology
University of Georgia
Athens, GA 30602-7229
=========================================
Phone: 706-542-1750
Fax: 706-542-3077
On Feb 22, 2007, at 1:23 PM, Douglas L. Theobald wrote:
Hi all,
I'd like to pick the collective brain of crystallographers on this
list -- what are some of the most easily crystallizable proteins?
I'm especially interested in those that over-express and diffract
well, and in ones that might be less well-known than, say, lysozyme
(but nearly as nice).
Douglas
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Douglas L. Theobald
Department of Biochemistry
Brandeis University
Waltham, MA 02454-9110
[EMAIL PROTECTED]
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