[ccp4bb] ATP binding

[Menachem Shoham]

Hello,
This is another off-topic question.
We are trying to purify a putative ATP-binding protein. What kind of  
beads or column would work best in this situation?
Jena Biosciences sells a kit of four different ATP-Sepharose beads  
with different attachment points of the ATP to the solid matrix. I  
wonder what experience people have with these columns.
What other solid ATP matrices are available?
What is the best way to demonstrate ATP binding prior to  
crystallization?

                                                                                
                                                                Thanks,
                                                                                
                                                                                
        Menachem Shoham

Menachem Shoham, Ph.D.
Case Western Reserve University
School of Medicine, Department of Biochemistry
10900 Euclid Ave
Cleveland, OH 44106-4935
Tel. 216-368-4665
Fax  216-368-8741
E-mail: [EMAIL PROTECTED]
http://www.cwru.edu/med/biochemistry/faculty/shoham.html