Hey Emmanuel

Typical R-factor for random structure will be above
57% and for initial MR model it will be somewhere
between 40-50%. In your case, High R-factor might be
due to many reasons. 


So check the data for any twining and also again check
the Space group.  Because sometime you will end up in
wrong space group due to twining also. Check for
twining using the following server

http://nihserver.mbi.ucla.edu/Twinning/

If you really confident on those, then check  the MR
solution and density map  to see is how the model fits
the density, that will give you more information.

hope this help.

cheers
VK

--- Emmanuel Prata <[EMAIL PROTECTED]> wrote:

> Dear all,
> 
> I have a data set of a protein soaked with sacarose,
> and the statistic
> data is good until the molecular replacement (Rmerge
> 0,07 (24%),
> I/Sigma 7 (2,7), completness (99,8 (98%)), and
> resolution 1,98A. After
> this step, I can not get the Rfactor (64%) to drop
> (I believe the
> spacegroup is correct).
> Could anyone give me suggestions?
> Thank you in advance,
> Emmanuel Prata
> 


Kabaleeswaran Venkataraman

Arise, Awake and stop not until goal is Reached 
                           -  Swami Vivekanand


 
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