Here's one: Mattos C, Rasmussen B, Ding X, Petsko GA, Ringe D. Analogous inhibitors of elastase do not always bind analogously. Nat Struct Biol. 1994 Jan;1(1):55-8.
Also, I had a similar story as a postdoc, soaking a poor, nonspecific peptide substrate into trypsin; it bound sort of backwards and a little out of register -- I never did get around to finishing refinement, depositing it, writing it up... I suspect that there are many more examples of ligands that bound "wrong" and never made it into publication, because people would rather spend their time pursuing other structures that look more as anticipated and seem more biologically meaningful. Evette S. Radisky, Ph.D. Assistant Professor and Associate Consultant II Mayo Clinic Cancer Center Griffin Cancer Research Building, Rm 310 4500 San Pablo Road Jacksonville, FL 32224 (904) 953-6372 (office) (904) 953-2857 (lab) -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Monday, January 22, 2007 6:30 PM To: CCP4BB@JISCMAIL.AC.UK Subject: : misbound ligand examples? A biochemist friend asked for examples of cases were a protein was co-crystallized with or soaked in a ligand that bound in the wrong place - say, because the ligand used wasn't quite the right one or because other important ligands were absent. I'm sure such examples are out there, especially when soaks were done at high concentrations, but I'm having trouble thinking of concrete examples. Help? thanks, Phoebe Rice ------------------------------------------------------------------------ --------------------------------------------------- Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 fax 773 702 0439 http://bmb.bsd.uchicago.edu/index.html http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
