The structure 1PMQ is a kinase that was crystallized with ATP derivative, and then soaked with an inhibitor. The ATP was displaced from the normal binding site, but directly contacts the inhibitor from the new binding site.
-- Kendall W. Nettles, PhD Assistant Professor Department of Biochemistry The Scripps Research Institute 5353 Parkside Dr. Jupiter Fl 33458 office 561-799-8851 fax 561-799-8805 cell 561-306-7566 > From: <[EMAIL PROTECTED]> > Reply-To: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> > Date: Mon, 22 Jan 2007 17:29:46 -0600 > To: <CCP4BB@JISCMAIL.AC.UK> > Subject: : misbound ligand examples? > > A biochemist friend asked for examples of cases were a protein was > co-crystallized with or soaked in a ligand that bound in the wrong place - > say, because the ligand used wasn't quite the right one or because other > important ligands were absent. > I'm sure such examples are out there, especially when soaks were done at > high concentrations, but I'm having trouble thinking of concrete examples. > Help? > thanks, > Phoebe Rice > > > ------------------------------------------------------------------------------ > --------------------------------------------- > Phoebe A. Rice > Assoc. Prof., Dept. of Biochemistry & Molecular Biology > The University of Chicago > phone 773 834 1723 > fax 773 702 0439 > http://bmb.bsd.uchicago.edu/index.html > http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
