Works with the primer3-1.1.3-candidate release. http://sourceforge.net/project/showfiles.php?group_id=112461&package_id=211061&release_id=578504
$ tar -xzvf primer3-1.1.3.tar.gz $ cd primer3-1.1.3/src/ $ make all && make test $ echo 'PRIMER_NUM_RETURN=11 PRIMER_TASK=pick_left_only SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG =' | ./primer3_core | tail -20 PRIMER_LEFT_8_SELF_ANY=2.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_LEFT_8_END_STABILITY=6.9000 PRIMER_LEFT_9_PENALTY=1.7978 PRIMER_LEFT_9_SEQUENCE=CACACACACACAGCACACAG PRIMER_LEFT_9=39,20 PRIMER_LEFT_9_TM=58.202 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_LEFT_9_SELF_ANY=2.00 PRIMER_LEFT_9_SELF_END=1.00 PRIMER_LEFT_9_END_STABILITY=6.7000 PRIMER_LEFT_10_PENALTY=1.8066 PRIMER_LEFT_10_SEQUENCE=TCCCGACCCAAATCGATAG PRIMER_LEFT_10=78,19 PRIMER_LEFT_10_TM=60.807 PRIMER_LEFT_10_GC_PERCENT=52.632 PRIMER_LEFT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_LEFT_10_END_STABILITY=5.6000 = $ echo 'PRIMER_NUM_RETURN=1000 PRIMER_TASK=pick_left_only SEQUENCE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG =' | ./primer3_core | tail -20 PRIMER_LEFT_70_SELF_ANY=3.00 PRIMER_LEFT_70_SELF_END=2.00 PRIMER_LEFT_70_END_STABILITY=5.7000 PRIMER_LEFT_71_PENALTY=8.4850 PRIMER_LEFT_71_SEQUENCE=CACACACACAGCACACAGGTATTAGT PRIMER_LEFT_71=41,26 PRIMER_LEFT_71_TM=62.485 PRIMER_LEFT_71_GC_PERCENT=46.154 PRIMER_LEFT_71_SELF_ANY=3.00 PRIMER_LEFT_71_SELF_END=2.00 PRIMER_LEFT_71_END_STABILITY=5.7000 PRIMER_LEFT_72_PENALTY=8.4850 PRIMER_LEFT_72_SEQUENCE=ACACACACACAGCACACAGGTATTAG PRIMER_LEFT_72=40,26 PRIMER_LEFT_72_TM=62.485 PRIMER_LEFT_72_GC_PERCENT=46.154 PRIMER_LEFT_72_SELF_ANY=3.00 PRIMER_LEFT_72_SELF_END=1.00 PRIMER_LEFT_72_END_STABILITY=5.9000 = -- primer3 does not output complete primer name if only choosing left or right primers https://bugs.launchpad.net/bugs/191053 You received this bug notification because you are a member of Ubuntu Bugs, which is subscribed to Ubuntu. -- ubuntu-bugs mailing list ubuntu-bugs@lists.ubuntu.com https://lists.ubuntu.com/mailman/listinfo/ubuntu-bugs
