ot judge only from one angle. You could of course try to fit it in and see
how it looks.
Cheers,Arne
On Mon, Jul 20, 2020 at 6:17 PM samer halabi
<30c2162795b2-dmarc-requ...@jiscmail.ac.uk> wrote:
Hello all,
I have few blobs in an MHC II structure I am working on, especially opposi
,Samer
On Monday, July 20, 2020, 05:39:23 PM GMT+1, Nils Marechal
<4954a024d277-dmarc-requ...@jiscmail.ac.uk> wrote:
Dear Samer,
What king of cryo-protectant did you use ?
Such a bent density, with that size, looks like an ethylene-diol.
Best regards,
Nils Marechal De :
Sent: Monday, July 20, 2020 5:27:37 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] electron density close Histidine side chain Dear Samer,
What king of cryo-protectant did you use ?
Such a bent density, with that size, looks like an ethylene-diol.
Best regards,
Nils Marech
ty
13 Oak Drive
Hamilton, NY 13346
email: rrowl...@colgate.edu
On Mon, Jul 20, 2020 at 12:17 PM samer halabi
<30c2162795b2-dmarc-requ...@jiscmail.ac.uk> wrote:
Hello all,
I have few blobs in an MHC II structure I am working on, especially opposite to
Histidine as in the accompanying s
Dear All,I am working on structures where Methionine is important in binding of
peptides to the MHC protein complex.Would anyone kindly like to share their
knowledge about anything they find it important about this particular amino
acid structurally? Sharing a paper or just few comments will be
o acid can), which allows this domain to access
conformational space, and apparently is important for dimerization. It does not
directly answer your question, but seems an interesting read.
Best regards,
Sahil Batra
On Fri, Aug 7, 2020 at 5:44 PM samer halabi
<30c2162795
flexible side chains of Methionines of the receptor protein (SRP54/Ffh or Get3)
can accommodate a variety of hydrophobic substrates in the protein binding
clefts.
I hope this helps you.
All the best,
Pascal
On Fri, Aug 7, 2020 at 5:14 AM samer halabi
<30c216279
surface and it wasn't too picky about the nature of the other
surface.
https://jvi.asm.org/content/91/2/e01085-16
Best of luck!
-Jesscia
On Fri, Aug 7, 2020 at 7:40 AM samer halabi
<30c2162795b2-dmarc-requ...@jiscmail.ac.uk> wrote:
Hello Rachelle,Many thanks for sharing the link a
Dear All,Sorry to be spamming you all.However, I would greatly appreciate if
you please could let me know only if you had recently experienced the same
problems with not being able to secrete a protein complex (that you used to be
able to secrete) using HighFive cells using Lonza insect xpress
Dear All,The lab of Prof. Jim Kaufman at the University of Edinburgh is looking
for a Research Technician to help us understand how chicken MHC molecules have
such profound effects on resistance and susceptibility to
economically-important diseases. We will begin by using protein expression
sys
Dear All,Sorry for disturbing you all with my current inquiry. However, I am in
the process of designing and expressing soluble proteins and I thought to ask
for your valuable advice.
1-What would be your first choice (protein-based) fluorophore to link it to the
soluble protein at its C-terminu
Dear All,Sorry to be disturbing you all, however the valuable input I get each
time I have an inquiry from the mailing list members, encourages me to inquire
again.
Do you know of (or use) a Fab with high-affinity of binding to its epitope (KD
in the Picomolar pM or the Femtomolar fM range), whe
,
Danton
From: CCP4 bulletin board on behalf of samer halabi
<30c2162795b2-dmarc-requ...@jiscmail.ac.uk>
Sent: December 12, 2021 10:31 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] High-affinity binding Fab to a known epitope amino acid
sequence Dear All, Sorry to be disturbing y
Dear All,
Sorry to be disturbing you with my new inquiry.
I always got great and trustworthy results using FUGUE (Protein homology
recognition using environment-specific amino acid substitution tables:
https://mizuguchilab.org/fugue/prfsearch.html) I use an amino acid sequence of
a viral protei
Dear All,
Sorry to be disturbing you all with my inquiry. I am tagging a membrane protein
(MHC I) just after the Signal peptide with either HA-tag or Myc-tag and
expressing it in mammalian cells. I am able to detect that protein by
immunoprecipitation followed by Western blotting using anti-Myc
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