Dear all,
I solved a protein crystal structure which is about 95KD and was purified from
pig liver. Based on the crystal structure, I missed 36 residues at its
N-terminus and 16 at its C-terminus. I tried to do N-terminus sequencing to
identify if these 36 residues was cleaved or not. However,
Dear all,
Sorry for bothering you. I have an off-topic question about how to choose a
suitable expression vector and strain to overexpress mitochondria protein in E
Coli for the future crystallographic study.
It is a human metabolic enzyme expressed in the liver. My colleagues tried to
overexp
Dear friends,
Thank you very much for your suggestions which are very helpful. I am writing
to thank you from the bottom of my heart. Here is the summary of the topic.
Best
Wei
#
QUESTION:
I solved a protein crystal structure which is about 95KD and was
Dear all,
I am sorry to bother you with two off-topic questions.
Question one:
I have solved a pig mitochondrial protein crystal structure whose molecular
weight is about 95kd. However, I do not have the sequence information of this
protein. It is weird that I could not blast its sequence in p
From: Frank von Delft [mailto:[EMAIL PROTECTED]
Sent: Mon 5/12/2008 11:12 AM
To: Yong, Wei
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Two off-topic questions
> only tried once). Does any body have ideas about how to extract mRNA from pig
> live (or animal tissue in general)?
>
Dear all,
I have a problem about HKL2000. I can start HKL2000 and load my data. I can
only open images ending with .img and process them. However, osc images could
not be opened and processed. There is no error message from HKL2000 main
window. I can not figure out what is wrong with it. Can an
Dear all,
I know that there are a lot of experts having experience in expressing a big
protein in E.coli or yeast. My project is about a 95kd covalently-FAD-binding
protein (Human protein). I tried very hard but have a bad luck over the past
1.5 years. I list what I did briefly so far. I am loo
