Sorry that I missed a letter. I wanted to extract mRNA from pig liver. Thank you, Frank, for having taught me a lesson. I will carefully check my emails before I send them to ccp4bb. I am looking forward to getting suggestions. Best wishes Wei Yong
________________________________ From: Frank von Delft [mailto:[EMAIL PROTECTED] Sent: Mon 5/12/2008 11:12 AM To: Yong, Wei Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Two off-topic questions > only tried once). Does any body have ideas about how to extract mRNA from pig > live (or animal tissue in general)? > From pig live? Yeah sure, toss it in a blender -- you can tell whether it's alive by the squealing. You may need quite a large blender, though, and a crane to lift it, pigs are deceptively large. It's trickier to keep the pig alive even after extracting the mRNA. The subject was dealt with at some length as far back as the late 16th century by a well-known English dramatist, although he framed the problem in more general terms. But do you really need a pound of the stuff? phx.