Re: [ccp4bb] completeness in scala

2012-05-15 Thread Toth, Eric
In sports, maximal effort is considered to be 110%, so you're actually 9.9% short of getting everything you could out of that crystal at its resolution limit. All things considered, that's not bad. _ Eric A. Toth, Ph.D. Assistant Professor Departme

Re: [ccp4bb] Spots not getting indexed properly in protein-DNA complex

2013-08-01 Thread Toth, Eric
That looks like a garden variety mosaic/kinda-crappy crystal. Without being there, it's impossible to tell if it was morphology (e.g. cracks and divots, etc.), crystal handling, or just bad luck. The bottom line is you need better data. The good news is that those crystals diffract to a reasonab

Re: [ccp4bb] Issue with Molecules per Asymmetric Unit for Molecular Replacement

2014-05-15 Thread Toth, Eric
Have you tried fixing the molecule that looks correct and searching for others? You might have greater than one but less than 9 molecules per ASU. When you do this, try imposing severe restraints on the packing function. This worked for me in Phaser with a difficult case. My anecdotal experience

[ccp4bb] Postdoctoral Position at the Institute for Bioscience and Biotechnology Research

2014-12-02 Thread Toth, Eric
Dear crystallographic community The Institute for Bioscience and Biotechnology Research (IBBR) at the University of Maryland is seeking a highly motivated postdoctoral fellow. We are seeking an individual with an interest in protein engineering and structural biology to participate in a new I

Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

2017-07-21 Thread Toth, Eric
With this type of behavior, one suspicion is that you didn’t let the reaction come to equilibrium prior to taking the measurement and the variability between time of mixing and measuring between individual replicates is introducing extra variability. Take a concentration at which you know there