In sports, maximal effort is considered to be 110%, so you're actually 9.9%
short of getting everything you could out of that crystal at its resolution
limit. All things considered, that's not bad.
_
Eric A. Toth, Ph.D.
Assistant Professor
Departme
That looks like a garden variety mosaic/kinda-crappy crystal. Without being
there, it's impossible to tell if it was morphology (e.g. cracks and divots,
etc.), crystal handling, or just bad luck. The bottom line is you need better
data. The good news is that those crystals diffract to a reasonab
Have you tried fixing the molecule that looks correct and searching for others?
You might have greater than one but less than 9 molecules per ASU.
When you do this, try imposing severe restraints on the packing function. This
worked for me in Phaser with a difficult case. My anecdotal experience
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With this type of behavior, one suspicion is that you didn’t let the reaction
come to equilibrium prior to taking the measurement and the variability between
time of mixing and measuring between individual replicates is introducing extra
variability. Take a concentration at which you know there