[ccp4bb] resolution limit stuck in Refmac5

Hi all, I'm working with good quality relatively complete x-ray diffraction data collected to a resolution limit 2.6 Å from a crystal of a protein with a small molecule ligand bound.  I ran MR from 10-4 Å and then did maximum likelihood rigid body refinement in Refmac5 against data from 50-3.

[ccp4bb] Academic Position in San Diego, California

a large urban public university in a lovely part of the planet. Please see attached advert or go to: http://www.bio.sdsu.edu/ and click on Faculty Positions Available==>Structural Biology Best wishes, Tom Huxford. Department of Chemistry & Biochemistry San Diego State University

Re: [ccp4bb] crystallization tips for acidic proteins

, Tom Huxford. San Diego State University LEGAL DISCLOSURE: The author of this post does not own shares in PHOSPHATE nor will he benefit financially from your use of PHOSPHATE as a crystallization screening reagent. Past performance does not insure future success. Blah blah blah... On Jul 1

Re: [ccp4bb] peptide for co-crystallization

more details. Tom Huxford. Department of Chemistry & Biochemistry San Diego State University On Aug 27, 2008, at 10:04 AM, Daniel Jin wrote: Hi,   We would like to order two peptides for co-crystallizaiton. I notice that there is a big price jump for peptide over 95% pure and over 98%

[ccp4bb] temporary files in CCP4i/Refmac

prise Dell Workstation. Thank you, Tom Huxford. Department of Chemistry & Biochemistry San Diego State University

[ccp4bb] Academic Position at San Diego State University

of the planet. Best wishes, Tom Huxford. ========== Tom Huxford. Structural Biochemistry Laboratory Department of Chemistry & Biochemistry San Diego State University (619) 594-1606

Re: [ccp4bb] 60 kDa contamination in Rosetta cells

(overnight). Tom Huxford. == Tom Huxford Structural Biochemistry Laboratory Department of Chemistry & Biochemistry San Diego State University (619) 594-1606 > On Jul 11, 2021, at 11:45 PM, Dilip Badgujar wrote: > > Greetings, everyone > I am trying to co-express two ma

Re: [ccp4bb] 60 kDa contamination in Rosetta cells

el it does not look like your 37 and 15 kDa bands are co-eluting. And your lane labels in the figure are not right. Good news: this seems like a solvable problem. Keep after it! Tom Huxford. ====== Tom Huxford Structural Biochemistry Laboratory Department of Chemistry & Biochem

[ccp4bb] Crystal with ZERO diffraction

responses. And happy holidays to us all. Tom Huxford. ====== Tom Huxford. Structural Biochemistry Laboratory Department of Chemistry & Biochemistry San Diego State University (619) 594-1606

Re: [ccp4bb] mystery blob!

Dear Eleanor, Perhaps a PMSF-EG adduct? Tom Huxford. == Tom Huxford Structural Biochemistry Laboratory Department of Chemistry & Biochemistry San Diego State University (619) 594-1606 > On Jun 26, 2024, at 8:09 AM, Eleanor Dodson > <176a9d5ebad7-dmarc-requ...@j

Re: [ccp4bb] unknown density

Dear Michael, It appears to me like a PEG molecule with two alternative conformations at its terminus and a bound water molecule? Nice maps! Tom Huxford. == Tom Huxford Structural Biochemistry Laboratory Department of Chemistry & Biochemistry San Diego State University (619)