Re: [ccp4bb] Using EMAN2.

2022-03-22 Thread Tom Calcraft
Hi Gurudatt, If the alignment of the tilt series is already good enough (this also applies to .ali stacks from IMOD), you can run eman2 tomogram reconstruction using the —noali and —niter=0 options, which will just reconstruct your tilt series in their current alignment. Note that the tilt axis

Re: [ccp4bb] Topology diagram

2024-05-31 Thread Tom Calcraft
Dear Lionel, If you want a programme that takes a PDB file and automatically generates a topology diagram, then Pro-origami may be the software you need (https://sites.google.com/site/alexdstivala/home/pro-origami) - it generates prettier diagrams than PDBsum, and the diagrams are output as .sv