Hi Gurudatt, If the alignment of the tilt series is already good enough (this also applies to .ali stacks from IMOD), you can run eman2 tomogram reconstruction using the —noali and —niter=0 options, which will just reconstruct your tilt series in their current alignment. Note that the tilt axis should lie along the Y axis in the aligned stacks.
Also, this question would be better addressed to the EMAN2 Google group (https://groups.google.com/g/eman2), or alternatively the 3dem or ccpem mailing lists. Best regards, Tom On 22 Mar 2022, at 13:53, Gurudatt Patra <gurudatt1...@gmail.com<mailto:gurudatt1...@gmail.com>> wrote: External Sender: Use caution. Dear All, I was wondering if we can use the aligned tilt series that we get from Serial EM as raw data directly for reconstruction and particle picking in EMAN2. Thank you so much in advance. Sincerely, Gurudatt ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=04%7C01%7C%7Cfe8c46f426314169310308da0c0ce6f8%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637835546821567058%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=DSfRY6zf0AAqgUmYlXc4eznI7XT8zMrNGjNruRxqNyU%3D&reserved=0> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
