Re: [ccp4bb] emergency substitute for RT loop cover?

The quality of glass and thickness may be a problem with the hematocrit caps but definitely worth a try in a pinch. - todd From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Prince, D Bryan [dbryan.pri...@astrazeneca.com] Sent: Monday, July 07

[ccp4bb] monomer/dimer protein

Hello All- I am interested in monomer/dimer contamination when building a crystal lattice, ie. if you are building a crystal lattice with a monomeric species of protein, incorporation of dimers may yield lattice or surface defects. This species may be considered a macromolecular contaminant. I

Re: [ccp4bb] P31 or P32

I agree here. The 50 % could be a good indicator. I am finishing a new structure which was about 42% twinned in the monoclinic setting (b = ~90) for an Se-Met dataset. When I went to refine it with the native dataset, the twin fraction increased to 50%, figured something wasn't right. fortunate

[ccp4bb] non-specific disulfide bonds in crystal structures

Hello All- I have recently determined a domain structure of a larger protein. The structure shows a clear disulfide bond between two monomers in the asymmetric unit. I'm trying to figure out if this is an artifact of the crystal packing or has biological relevance. The protein has been reported

Re: [ccp4bb] active 3D monitors: successor of Asus VG278HR?

I'll second Jim on this. Just this week, I set up my linux box with the nvidia 3d vision 2 kit which I use with the ASUS VG278HE. My graphics card is the quadro K4000 and the only pitfall was that I had to purchase the 3 pin connector to get it to sync: http://www.cdw.com/shop/products/PNY-full

Re: [ccp4bb] 3BDN, 16.5% Ramachandran Outliers!!!!!

My guess is they had the best data they could get, did molecular replacement with the two halves of the repressor and the dna, got a solution and didn't use appropriate restraints in the refinement. Like Phoebe mentioned, we have better tools for this these days. __

Re: [ccp4bb] 3BDN, 16.5% Ramachandran Outliers!!!!!

wrote: The abstract of the papers says they used MIR. Mark J van Raaij Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij > > > > > > On 23 Apr 2015, at 18:

Re: [ccp4bb] Reindexing of lattice

I think if you chose option "entering reflection transformation" from the define transformation matrix button under reindexing details and then use: h->k, k->l, l->h i think you should be ok. -Todd From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of

Re: [ccp4bb] HKL2000 24IDE beam center.

If you change it in the def.site file, when you start HKL2000 and select the detector type, it has to be the one with the name of the directory of the def.site file that you corrected. If it doesn't have the values that you entered into the def.site file then you are either not choosing the corr

Re: [ccp4bb] first use of synchrotron radiation in PX

I never would have survived the dress code back then. From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Anastassis Perrakis [a.perra...@nki.nl] Sent: Wednesday, March 13, 2013 2:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] first use