Hello All- I am interested in monomer/dimer contamination when building a crystal lattice, ie. if you are building a crystal lattice with a monomeric species of protein, incorporation of dimers may yield lattice or surface defects. This species may be considered a macromolecular contaminant. I have read a few papers on this subject, a couple are listed here:
I. Yoshizaki et al. / Journal of Crystal Growth 290 (2006) 185–191 Caylor CL1, Dobrianov I, Lemay SG, Kimmer C, Kriminski S, Finkelstein KD, Zipfel W, Webb WW, Thomas BR, Chernov AA, Thorne RE. Proteins. 1999 Aug 15;36(3):270-81. In each of the studies that I have read, lysozyme is the model protein for these studies. I have not seen studies thus far that have been done with other proteins. Can anyone point me toward other studies, specifically non-lysozyme studies, where incorporation of two different oligomerization states has been shown to yield crystals with higher level of defects? Microscopy studies of such would be great too. Thanks in advance- Todd
