[ccp4bb] Methylation of macromolecular complexes

contributions by lysines might be a cause for not obtaining crystals, or is there any other better ways? thanks, Jan Rash

Re: [ccp4bb] Methylation of macromolecular complexes

Hi all, What I really want to know is whether macromolecular complexes ( more than 200kDa) can tolerate methylation? thanks, Jan Rash 2009/11/24 Hüsnü Topal > Hi Jan, > > > > Maybe this link > http://www.jenabioscience.com/cms/en/1/catalog/529_jbs_methylation_kit.htmlor

[ccp4bb] crystallization of a macromolecular complex

Dear All, This is about the crystallization of the macromolecular complex which is highly soluble and shows no signs of the aggregation (even at high concentration). We have tried several salts, precipitants and even high protein concentration (around 20g/L) for its crystallization without any gen

[ccp4bb] Protein-antibody complex

Hi All, I have a simple question about the complex formation between macromolecular complex and antibody. My protein is stable in the presence of the 5mM DTT and under these conditions the reducing environment is too strong for the antibody to survive. I am also now trying to check the stabilit

[ccp4bb] Crystallization of lysine and arginine rich proteins

Dear All, I have a question regarding the crystallization of lysine and arginine rich protein around 13%. So far our attempts to crystallize this protein have not been successful although the secondary structure predictions, CD spectroscopy measurements clearly show that this protein is folded. I