[ccp4bb] refine phosphotyrosine

Hi,   I am trying to refine a peptide with phosphotyrosine in CNS. I downloaded the parameter and topology files for phosphotyrosine from the hiccup database and pasted into the protein.param and protein.top files from the CNS library. But I am not able to run generate.inp with these modified

[ccp4bb] restrained refinement in Refmac

Hello all, I'm working on a protein-DNA complex. My protein is a trimer and the crystal has 3 trimers in an AU. I used a pdb file including three subunits as a search model when I did molecular replacement in CCP4. I did a rigid body refinement in Recfmac the R-factor and R-free did not go d

[ccp4bb] Malic Acid interferes heavy metal binding?

rystals grow with similar conditions? Any comment and suggestion is highly appreciated. Regards, Jinjin Zhang

[ccp4bb] 2fo-fc and fo-fc

with it? Thanks. Best, Jinjin Zhang

[ccp4bb] brom- / iodo- DNA source?

Dear all, Where is a good place to order brom-cytosine or iodo-dC? Thanks. Best, Jinjin Zhang

[ccp4bb] 27.5% R/29% Rfree for 1.75A structure

Hi All, We have pretty much finished fitting and refining a protein-peptide complex structure, however, the 27.5% R/29% Rfree seem quite for a 1.75A resolution data. Does anybody have some idea about this kind of problem? Thank you. Best,Jinjin Zhang

[ccp4bb] 27.5% R/29% Rfree for 1.75A structure (details updated)

 is suspected. 5. Water molecules added Thanks a lot for all your comments and suggestions. Best, Jinjin Zhang

[ccp4bb] 27.5% R/29% Rfree for 1.75A structure (details updated)

 is suspected. 5. Water molecules added Thanks a lot for all your comments and suggestions. Best, Jinjin Zhang