Hello,
I am trying to reproduce two hits obtained from the PEG/Ion screen of
Hampton which showed up with 20% PEG3350 and 0.2M K2HPO4/0.2M CaCl2. I
tried optimizations making up my own solutions with the two salts from Sigma
(The PEG is from Hampton). To my surprise, the hits do not translate
Thanks all for the suggestion. I have ordered the additional salts from
Hampton. I still have about 600uL of the original solution in the block. BTW,
the hits obtained were in two different conditions with the two salts.
They were PEG/Ion HT screen A7 and D6.
I am keeping my fingers crossed.
Sin
Hello
I am designing a protein construct for structural biology. It is a protein
kinase
which has not been crystallized earlier. I was comparing the kinase domains of
other closely related family members characterized biochemically vs
crystallization constructs. For crystallography constructs,
