[ccp4bb] Cannot select any recommended SG for the protein BsAlaDH

2023-08-02 Thread CENGIZ KAAN FERAH
Hello, So I'm trying to get the data processed that I gathered from XRD for the protein BsAlaDH. Unfortunately from the method that I know of on CrysAlisPro I cannot select the recommended space group for the protein. This results in the data not being complete. Still I can get good unit cells and

[ccp4bb] Soaking my protein crystals with its substrate

2025-03-17 Thread CENGIZ KAAN FERAH
Hello everyone, I'm currently working on a protein and I did solve the apo structure of the enzyme. I'm trying to get the ligand bound form of the enzyme. The ligand is L-alanine. How should I soak with the ligand? I'm doing microbatch under oil with a terasaki plate without any machinery. Thank

[ccp4bb] Unit Cell Volume Confusion

2025-02-28 Thread CENGIZ KAAN FERAH
Hello, There's something that I'm currently struggling with in terms of Molecular Replacement and Refinement. From my mtz file, my solutions unit cells are consistent with PDB entries of homologous structures. But when I'm trying to get fit in the same oligomeric structure which is hexameric it do