pdb header.
Regards, Ben
Ben Bax
Senior Scientific Investigator
BioMolecular Sciences UK
RD Platform Technology & Science
GSK
Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK
Email benjamin.d@gsk.com<mailto:benjamin.d@gsk.com>
Mobile +44 (0) 7912
(and protonated)
forms of AMPPNP the same. When I tried to deposit a specific AMPPNP tautomer in
2013, they would not accept it. The PDB also seems to believe, as I understand
it, that the overall charge on AMPPNP is zero and that the phosphates do not
carry negative charge.
Ben Bax
Senior
The twin fraction can vary during data collection - if the beam is smaller than
the crystal
For example you could have crystals which are like two very short ends of
pencils - with flat ends together (in your case these might pencils could be in
P3)
In some orientations you could have the beam g
Hi Jorge,
I have had some similar cases.
The twin fraction can vary during data collection on the synchrotron
(different areas of the crystal can have different twin fractions).
Sometimes this is not a problem and twin refinement works fine - sometimes
it is a problem and it is hard to get a cle
Hi Kavya,
More than one crystal?
Epitaxial growth (protein crystal growing on salt crystal or vice versa)
Did you try IZIT?
(https://hamptonresearch.com/product-Izit-Crystal-Dye-33.html).
I have seen salt and protein crystals in the same drop before now - but not
growing together.
Ben
On 3
Fcalc maps look fantastic. Are you sure they were not using an Fcalc for the
missing 35% of the data?
Ben
On 29 Feb 2024, at 21:33, Rafael Marques wrote:
Sorry for jumping into the post, but I would like the community’s opinion about
completeness, once this topic was raised here. W
Hi,
I am just re-refining an old twinned dataset - which I will
redeposit (refinement is never finished).
The pdb seem to have automagically detwinned my data.
Where do I find my original twinned data (F and SIGF)? (or will I have
to redeposit my original un-detwinned structure factors).
Tha
Dear CCP4BB,
I am refining a structure crystallised in the presence of Jeffamine 600.
The CCP4 dictionary for Jeffamine says all nine chiral atoms have a
defined chirality.
Is this correct or not?
Thanks, Ben Bax
loop_
_chem_comp_chir.comp_id
_chem_comp_chir.id
ltration assay that ran on 6uls of protein
(automatically injected from a 96 well plate). This assay was used to
optimize the length of the DNA, the DNA sequence, and the metal ions
used - it is described in the supplementary material to the Nature
article.
Best regards, Ben Bax
On Tue, Dec 17, 2
