Re: [ccp4bb] Diffraction data - Midwest Center for Structural Genomics (MCSG) structures

Dear All, This bulletin is truly amazing, thank you for all the messages and replies! I am already in contact with the people in charge of the project that superseded MCSG (Andrzej Joachimiak and Karolina Michalska). Thank you also to Changsoo Chang that found the data and is about to send

[ccp4bb] 7th International Symposium on Diffraction Structural Biology ( 5 - 7 May 2025)

Dear Colleagues, We are delighted to announce the *Scientific Programme *for the *7th  International Symposium on Diffraction Structural Biology (ISDSB2025) is now out! *The event will take place from *5–7 May 2025* at the *European Photon and Neutron Science (EPN) Campus in Grenoble, France,

Re: [ccp4bb] Diffraction data - Midwest Center for Structural Genomics (MCSG) structures

Dear all, at the bottom of https://staraniso.globalphasing.org/table1/ you'll find a (weekly updated) page that associates PDB entries with (hopefully) raw diffraction data. Cheers Clemens On Mon, Mar 17, 2025 at 03:02:33PM +, Jose Artur Brito wrote: > Dear All, > There are several str

Re: [ccp4bb] About generation of restraint file

On Tue, 18 Mar 2025 09:21:44 +0100 Christian Buratto wrote: > Dear CCP4 community, > I am currently trying to determine the structure of a > protein-polyoxometalate complex. I am using a polyoxometalate > molecule that is a derivative of the one deposited in the CCDC (ICSD: > 173439). I am facing

Re: [ccp4bb] Dose-dependent evolution of the cryo-EM density of the disulfide bond (Re: [ccp4bb] Query Regarding Additional Density Near Disulfide Bond in Cryo-EM Structure

Ah that's right, I had forgotten about this supplementary video. I even read this paper again not too long ago. It's working better than the HexAuFoil paper suggests it should, that's interesting to see. But it might not be generally applicable to all datasets? especially of lower resolution. _

Re: [ccp4bb] Query Regarding Additional Density Near Disulfide Bond in Cryo-EM Structure

Thank you Wim, this is an interesting read. Do you also have a reference about radiation damage in electron microscopy? From: CCP4 bulletin board on behalf of Wim Burmeister Sent: Monday, March 17, 2025 5:27:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb]

[ccp4bb] About generation of restraint file

Dear CCP4 community, I am currently trying to determine the structure of a protein-polyoxometalate complex. I am using a polyoxometalate molecule that is a derivative of the one deposited in the CCDC (ICSD: 173439). I am facing difficulties in generating a restraint file for this metal-oxo molecule

[ccp4bb] Spreadsheet DoE for optimising protein production & characterisation

Hi all, Please find here: https://zenodo.org/records/13998442 a series of factorial design spreadsheets we developed to help your lab optimise protein expression, purification, crystallisation, stability etc. These are free to download and are intended to lower the barrier/cost for using this p

[ccp4bb] Change e-mail address

Hi, I am kindly enquiring whether it would be possible to change the email to which my ccp4i bulletin board emails get sent. Old e-mail address: doriano.la...@ic.cnr.it New email address: doriano.la...@cnr.it Thank you very much! Doriano Lamba

[ccp4bb] Reminder: CBMS Lecture Series - March 19, 13:30 (EST) - Bowman

You are cordially invited to join the Center for Biomolecular Structure Lecture Series … SARAH E. J. BOWMAN University at Buffalo Jacobs School of Medicine and Biomedical Sciences WEDNESDAY, MARCH 19, 13:30 (EST) "Shining light on metalloproteins: Multiple methods tell the story" Register

[ccp4bb] Register now for the triennial Ribosomes 2025 Conference at Asilomar June 22-27

Dear all, Registration for Ribosomes 2025 is now open. This 19th triennial international ribosome conference will take place at the Asilomar Conference Ground in Pacific Grove, California, from June 22-27, 2025. The scientific program will feature talks and posters on the structure and functi