We bought some recently hoping for some magic for a specific project
that we needed new crystal forms for. Alas, no fairy dust magic in our
hands. Maybe we didn't do the protocol right - but of course, one
doesn't expect to have to do that with fairy dust. We haven't tried with
many things, t
[cid:image001.png@01D3C766.E1037C50]
We're pleased to announce that registration is now open for SGC Oxford's
upcoming symposium "Exploiting Disease Genomics to Catalyse New Medicines"
which will take place at St. Catherine's College, Oxford on 14th June 2018.
Full details and links for registr
Dear all,
I have an opening for a postdoctoral researcher in my group at the
Department of Cell and Molecular Biology, Uppsala University.
The project aims to study the structure and function of metal-responsive
transcription factors. The successful candidate should have an excellent
track r
Hi All,
I am writing to seek advice on doing sulphur SAD data collection
at Cu based home source for a protein that is 12 KDa and has 6 S atoms.
I have seen some links online and some references but would be grateful if
you can share your know-how for success with this.
Like what multiplicity of
Well - the S f" is only ~ 0.5 at Cu Kalpha so the signal will be very
weak..
Very accurate data may get a solution but you first have to position the S
atoms...
Much easier to try to make a heavy atom derivative!
Eleanor
On 3 April 2018 at 15:26, Manoj Saxena <
1d16aa30e8a1-dmarc-requ...@jis
Dear Manoj,
providing your crystals diffract to at least 2.5 Å and the sulfurs are
ordered, you should be able to solve your structure without too many
problems. As a first experiment, I would recommend collecting 360 degrees
of data. Process these with XDS and the FRIEDEL'S_LAW= FALSE option an
Iodine is ideally suited for Cu K-alpha SAD phasing, and iodide ions can
normally be easily added by soaking crystals in potassium iodide
containing solutions, which can be done at the time of cryopreservation.
A quick lit search will turn up the appropriate protocols. For
structural genomics w
Hi Manoj,
Like Eleanor has pointed out a very accurate data may get you a solution. If
you have crystals that diffract well and give you data with high I/sigma(I) you
can attempt to find the positions of the S atoms and use that to arrive at a
structure. Using lysozyme as a test case we have sh
Thank you!, Andreas and all others who replied.
I have seen your tutorial and that's what I was referring to.
Maybe I can consult you later when I screen some samples and have an idea
of signal strength?
Regards
Manoj
2018-04-03 10:55 GMT-04:00 Andreas Förster :
> Dear Manoj,
>
> providing you
If you merge data with AIMLESS I + and I - are always reported seperately
and it reports a CCanom checking whether two randomly selected data halves
have a correlation in the anom signal .
Often *IF* you can position the S atoms the phasing steps work, but it can
be challenging to get the sites
Dear Manoj,
I'm happy to answer your questions and have a look at your data. Maybe we
can take this off-list and you report back if and when you solve your
structure.
By the way, I'm processing native data collected with a MetalJet at the
moment. The anomalous signal is even weaker at 1.35 Å bu
Jim Pflugrath and a couple of high school interns shot a short video on halide
quick soaks a few years back. You can find it at
https://www.youtube.com/watch?v=45Qc3jOPaKY.
Cheers,
Joseph D. Ferrara, Ph.D.
CSO
Deputy Director, X-ray Research Laboratory
Vice President, American Crystallographic
Hi Manoj,
it depends on crystal and hardware (detector, beam, goniometer) quality as well
as on the correct strategy whether you will be able to solve the structure with
sulfur SAD. 6 sulfur in 12 kDa should be doable, if the crystals diffract to
2.5A or better. Others have given good advice or
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