Thank you!, Andreas and all others who replied. I have seen your tutorial and that's what I was referring to. Maybe I can consult you later when I screen some samples and have an idea of signal strength?
Regards Manoj 2018-04-03 10:55 GMT-04:00 Andreas Förster <andreas.foers...@dectris.com>: > Dear Manoj, > > providing your crystals diffract to at least 2.5 Å and the sulfurs are > ordered, you should be able to solve your structure without too many > problems. As a first experiment, I would recommend collecting 360 degrees > of data. Process these with XDS and the FRIEDEL'S_LAW= FALSE option and > see how much anomalous signal CORRECT.LP reports, if you've got stars in > the values of the Anomal Corr column, you're on the right track. Try to > phase with SHELXD, following the advice on http://shelx.uni-goettingen > .de/tutorials.php. > > If you don't succeed, you might need higher multiplicity. If you have a > kappa or chi goniometer, you can reorient the crystal and collect another > 360 degrees and another 360 degrees, and so on. Merge all data in XSCALE > until you can solve the structure. > > I once wrote a little tutorial for doing S-SAD on the home source that you > might want to follow. http://www.imperial.ac.uk/x-ra > y-crystallography/learning-more/sulphur-sad/ With an HPC detector (or > even an image plate) you'd getter better data than with a CCD detector > and be able to solve your structure quicker. (Disclaimer: I work for a > company that makes HPC detectors.) I consider the home source a great > place to solve structures by native SAD. > > All best. > > > Andreas > > > > On Tue, Apr 3, 2018 at 4:26 PM, Manoj Saxena < > 00001d16aa30e8a1-dmarc-requ...@jiscmail.ac.uk> wrote: > >> Hi All, >> >> I am writing to seek advice on doing sulphur SAD data collection >> at Cu based home source for a protein that is 12 KDa and has 6 S atoms. >> I have seen some links online and some references but would be grateful if >> you can share your know-how for success with this. >> Like what multiplicity of data would be good to aim for and >> data processing tips. >> Inputs from people who have tried and failed would also be highly >> appreciated. >> >> Thank you >> Manoj Saxena >> University of Puerto Rico >> >> >> > > > -- > <https://www.dectris.com> > Andreas Förster, Ph.D. > MX Application Scientist, Scientific Sales > Phone: +41 56 500 21 00 <+41%2056%20500%2021%2000> | Direct: +41 56 500 > 21 76 <+41%2056%20500%2021%2076> | Email: andreas.foers...@dectris.com > DECTRIS Ltd. | Taefernweg 1 > <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> > > <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g>| > 5405 Baden-Daettwil > <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> > > <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> > | Switz > <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> > erland > <https://maps.google.com/?q=Taefernweg+1+%C2%A0+%7C+5405+Baden-Daettwil+%C2%A0+%7C%C2%A0Switz+erland&entry=gmail&source=g> > | www.dectris.com > > [image: LinkedIn] <https://www.linkedin.com/company/5067919> > [image: facebook] > <https://www.facebook.com/pages/Dectris-Ltd/623855944369304> > <https://twitter.com/DECTRIS_News> > > > > > > Confidentiality Note: This message is intended only for the use of the > named > recipient(s) and may contain confidential and/or privileged information. If > you > are not the intended recipient, please contact the sender and delete the > message. > Any unauthorized use of the information contained in this message is > prohibited. > > >
