Hi Lingyuan
I would certainly make a seed stock and use it with RANDOM screens - this
will (usually) allow you to pick up new conditions and control the number
of crystals per drop.
However you say your crystals melt easily when you harvest them. I don't
know how David stabilized his seedstock (
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Dear All,
I would like to know if it is possible to use a low resolution EM
reconstruction of a complex obtained in negative stain EM (not cryo EM) to
help molecular replacement in a 4.5A resolution X-ray diffraction data set
of the same complex
I am aware of the possibility of using low resolutio
