Dear all, I have collected 2 datasets (space group P1, resolution 1.4) on a
bruker machine using Proteum2 v2014.9-0 software. I integrated both datasets
with SAINT and would like to continue with Pointless - SCALA - Truncate to
finally get*scaled but unmerged data* to be able to calculate C
Dear Colleagues,
After reading a few papers about growing suitable crystals for neutron
diffraction. I will do capillary counterdiffusion with an agarose plug
between mother liquor and protein solution like described in
http://www.ncbi.nlm.nih.gov/pubmed/23192028.
However I looked quite som
Hi Georg,
I haven't used the agarose method, but have sealed many capillaries using bees
wax without an issue.
Simply heat the wax until it melts, dip the capillary (rotate if it has a
larger diameter) then let cool.
I hope that helps, cheers!
Sean Seaver, PhD
P212121
http://store.p212121.co
Dear Georg,
Since you collected your data on a Bruker machine and integrated them
with SAINT you should simply scale the .raw files with the Bruker
program SADABS and then read them into into XPREP. This can scale the
two datasets together and produce either merged or unmerged but scaled
data
https://hamptonresearch.com/product_detail.aspx?cid=10&sid=65&pid=109
Granada box will work for you
Thaumatin can be grown to large single crystals suitable size
On Mon, Jan 5, 2015 at 9:25 AM, Georg Mlynek
wrote:
> Dear Colleagues,
>
> After reading a few papers about growing suitable crystal
As George says, you can do it all with Bruker programs
However, Pointless is supposed to read .raw files, but when I wrote the code
(at the request of a user) I didn't have complete information, so I may have
missed something. The error message is from somehow getting a zero or negative
batch
Hi,
Warmed up capillary and hamilton syringe works fine (i used heat lamps
similar to pizza heaters used by all-night pizza joints). You can get a
cheap heat lamp from most pet stores (terrarium heaters).
Artem
On Jan 5, 2015 11:32 AM, "Georg Mlynek" wrote:
> Dear Colleagues,
>
> After reading
Hello Georg,
You can melt the agarose in a glass container using a microwave oven. Then
connect your capillary tube to small syringe, using a plastic tubing or
adapter. Use some parafilm if the tubing diameters do not fit exactly,
there should be no leaks. Then slowly pull the plunger to take ~100
Dear Georg,
There are different ways to set-up counterdiffusion experiment but the
set-up you mentioned is done in a single capillary. The easiest way is to
prepare the agarose and when it is still warm pipette the 100 microL on any
surface, I use a piece of parafilm. Them you have to adsorb it wi
Dear George and Phil, thanks a lot for the fast answers. Things are
unfortunately a bit more complicated and the usually very convenient way using
SAINT-SADABS-XPREP has too much limitations for this datasets because
1. It starts with that one datasets has more than 2.000.000 reflections (space
Dear All,
When I run Refmac, it would produce a refined PDB file and mtz file. My
question is, if I started the refinement at 6:00 pm and completed at 8:00 pm, I
find the refined PDB file and mtz file were created in the target directory at
perhaps 6:30 pm. I think from 6:30 pm the created PDB f
Dear Dialing
are you talking about the 'creating date' or the 'modified date'?
Leo
> On Jan 6, 2015, at 2:55 AM, Dialing Pretty
> <03f1d08ed29c-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Dear All,
>
> When I run Refmac, it would produce a refined PDB file and mtz file. My
> question is, if
I am talking the "creating date". For my situation, once the PDB file and mtz
file were created at around 6:00 pm, with the progression of the refinement and
completed at 8:00 pm, the date shown in the directory folder is always 6:00 pm.
After 8:00 pm when the refinement finished, I check the pr
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