[ccp4bb] on modification the Cbeta of Ser

Dear All, Can anyone tell me how to change the position of Cbeta of Ser by Coot? Cheers,   Dialing

Re: [ccp4bb] [OFF-TOPIC] Site-Directed Mutagenesis

Hi Fred, While you are working on the optimization of your PCR, I strongly suggest you to read our paper on partial overlapping primer design from a link below, which will surely ease your mutagenesis: http://www.ncbi.nlm.nih.gov/pubmed/21530481 I would be happy to answer your questions sh

[ccp4bb] off-topic:schematic representations for secondary structure

Dear memebers, Thank you all firsit for the helps on my previous post about sequence alignment. They are really useful! Apologize for the off-topic question again. I usually use DSSP method to calculate the secondary structures on my sovled structures. I saw a nice schematic representation for s

Re: [ccp4bb] off-topic:schematic representations for secondary structure

I believe Procheck generates drawings such as those. It generates PostScript files, and if you need to have (eg) jpg files, a PostScript interpreter, screen capture and there you are (Gimp to select only the areas you're interested in) HTH, Fred. WENHE ZHONG wrote: Dear memebers, Thank yo

Re: [ccp4bb] Bio Rad "Econo" system

I've been using the Econo system for about 20 years for prep scale purifications. I regularly do gradients using an external gradient maker (something like with Hoefer sells - http://www.hoeferinc.com/product.asp?ProdID=171) that feeds into the pump. It's worked reliably for me and allows the