Hi, everyone,
I can get my protein complex but there are some non-specific aggregation
from the NMR spectra, and chaps can improve it.
So, besides chaps, is there any other detergents to be used during crystal
screening? All suggestions are welcome.
Thanks&Regards,
Yuan
Gregory Bowman wrote:
Yes, but I don't actually want to swap a and c (convention that a is shorter
than c), but instead flip k and keep h and l the same. Incidentally, it is not
immediately obvious to me why in matrix I cited below that the new l is h+l:
-1 0 0
0 -1 0
1 0 1
Greg
Because -a a
Sorry for adding confusion- of course in reciprocal space beta* should be
acute. The figures should have been labeled a, c not h,L.
Edward A. Berry wrote:
Gregory Bowman wrote:
Yes, but I don't actually want to swap a and c (convention that a is shorter
than c),
but instead flip k and keep h a
Hampton makes a "Detergent Screen" that works the same way as the Additive
Screen.
http://hamptonresearch.com/product_detail.aspx?cid=1&sid=39&pid=31
It has 96 unique detergent reagents for crystal screening.
It is a bit expensive, but if you don't want to purchase the whole kit you
could ju
Dear Andrea
check out:
Post-crystallization treatments for improving diffraction quality of protein
crystals.
Heras B, Martin JL.
Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1173-80.
All the best
Savvas
On 26 Aug 2011, at 22:53, Andrea L Edwards wrote:
> Hi all,
>
> What are the mos
Hi, Anita
If you could find a way to test the elute's activity/binding to its'
substrat/cofactor, then you will learn much more about your target. If the
function assay is elusive, you could try superose column (5KDa-5MKDa). Does
your light scattering tell you about the estimated size and MW?
Bes
