hi guys
I am trying to crystallize protein complexed with peptide(14mer).
In initial screening i got microcrystals in following condition
- 0.2M MgCL2
0.1M Tris pH 8.5
28 % PEG 4000
Ptotein concentration - 12.5mg/ml and peptide conc.-5mg/ml
Complex ratio- (Protein-Peptide)(1:1.5)
Incubation
Sure it's not the peptide that crystallizes ?
You could try adding glycerol, exchanging the PEG, lowering the PEG
playing with the pH or changing to a different buffer system.
You might have done all these already, then you could also try a larger drop
size.
You could also mix your protein with yo
