hi guys I am trying to crystallize protein complexed with peptide(14mer). In initial screening i got microcrystals in following condition - 0.2M MgCL2 0.1M Tris pH 8.5 28 % PEG 4000 Ptotein concentration - 12.5mg/ml and peptide conc.-5mg/ml Complex ratio- (Protein-Peptide)(1:1.5) Incubation temp.-22 degree Method - Sitting drop vapor diffusion method
within four hours of setting trials I can see too many microcrystals. Though i tried reduction of protein and precipitant conc. as well as using mineral oil to decrease evaporation rate but still it is not working. I am waiting for your valuable suggestions.
