In light of several wonderful responses,I would like to provide an
update for this question:
1. I would agree that SEC might not be able to identify monomer vs
multimer forms for this likely rod-shaped protein.
2. It is extremely low kd for dimer. AUC and SAXS experiments have
measured it
My deepest and heartfelt condolences to Elspeth and her family at this
very difficult time. May his soul rest in peace.
Raji
---
Raji Edayathumangalam
Joint Research Fellow
Harvard Medical School/
Brigham and Women's Hospital
Brandeis University
On Jul 3, 2010, at 6:28 AM, Frank von
It's an interesting discussion.
1.Usually it's not possible to use mass-spec for non-covalent complexes.
2. Most methods depend on macromolecule shape and concentration.
3. SAXS method looks limited to me. It uses diluted monodisperse solutions.
That excludes complexes that can associate/diss
4. The physiological concentration is a bit misleading. First, its clear
now that cells have microenvironments,
and 'physiological' concentrations are hard to define. Also, in a cell, I
think (and I think others tend to agree)
that kD plays little role at the end. kD is a combination of k(on) -
