[ccp4bb] Elspeth Garman's husband

2010-07-03 Thread Frank von Delft
Dear all Last night, John Barnett, physicist and husband of Elspeth Garman, passed away after a long battle with cancer. Please join me in passing condolences to Elspeth and her family. She mentioned that she may not be particularly responsive in the next while. Frank

Re: [ccp4bb] attachments

2010-07-03 Thread Kay Diederichs
As I'm the one who administers the CCP4 user wiki ( http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Main_Page ) I'd say that yes, the wiki is made for this, and yes, disk space is not a problem (within reason), so somebody should go ahead and create a new article "Problematic densit

[ccp4bb] monomeric coiled coil

2010-07-03 Thread aidong
Sorry for this ccp4 unrelated question. We recently have a protein that a multicoil program (http://groups.csail.mit.edu/cb/multicoil/cgi-bin/multicoil.cgi/cgi-bin/multicoil ) predicts to have very high probability for dimer and trimer. Their scores are close to 0.4 and 0.6 for lengths of mor

Re: [ccp4bb] attachments

2010-07-03 Thread Kay Diederichs
Hmmm, after thinking some more about this I need to add: my intention in running the CCP4 user wiki is not that it should be a dump site for random stuff. So please don't consider the wiki as an upload site for a file, just for sending its URL to CCP4BB, and to forget about it afterwards once t

[ccp4bb] Another vote for PINE.

2010-07-03 Thread Robert Sweet
One can keep ones hands on the keyboard to do everything -- no reaching for the mouse. An added advantage is that to delete a message doesn't reqire one to find and select a small check box with the mouse cursor, then to find and select the Delete check-box, which often has scrolled off the to

Re: [ccp4bb] monomeric coiled coil

2010-07-03 Thread chern
The multimeric state depends on a protein concentration. You can get any multimer to dissociate if you dilute it to low enough concentration. If your complex is a homodimer, then Kdiss=[complex]/[monomer]^2. Let's say your Kdiss~10^(-3)M, and your protein concentration is ~10^(-4)M, then [com

Re: [ccp4bb] monomeric coiled coil

2010-07-03 Thread Anastassis Perrakis
A few thoughts on these, since I do not fully agree. 1. Detection by light scattering is a method that can be used either without separation, or while separating. If you have a scattering detector, you can stick in a cuvette, or stick it to the end of a column, your choice. 2. Sec is not a

Re: [ccp4bb] attachments

2010-07-03 Thread Javier Gonzalez
Dear all, I've just created a group on Facebook named "CCP4 Stuff". This would be a good place to upload as much random stuff as you want (images, videos, links, comments, etc) that nobody will care about after a couple of days. All you need is to join (this is not an issue, considering that mos