Re: [ccp4bb] Pictures of DDM crystals?

Hi Joe, It is often useful to prepare in parallel drop without protein but with a large excess of detergent and protein buffer: a typical membrane protein of ca 100 kDa will bind something like 100-200 molecules of DDM, If you want to match the quantity of detergent that is bound to a 10 mg/ml

[ccp4bb] AW: [ccp4bb] Disulfide bond "survival" in the presence of DTT?

Hi Brad, For DosR, a stable disulfide bond was reported even in presence of 150 mM DTT - was reduced only after Thermal denaturation. see J Mol Biol. 2008 Nov 21;383(4):822-36 Best Clemens Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von Brad Bennett Gesend

Re: [ccp4bb] DNA binding protein

You can run a few µl of your sample on a native gel or agarose gel and visualise co-purified DNA by ethidium bromide/gel red staining. If your protein is stable at high salt concentrations (and a lot of DNA-binding proteins are) you can use a high salt concentration (like 1 M) in the lysis buff

[ccp4bb] Practical Course for Macromolecular Crystallography M2M-2009

This is a gentle reminder that 30 days is left until the deadline for applications to the Practical Course on Training in methods for Macromolecular Crystallography M2M-2009: From Measurement to Model. The course will take place at the EMBL Hamburg Outstation, the DESY synchrotron site during t

[ccp4bb] Postdoctoral Positions

Hauptman-Woodward Medical Research Institute Post-doctoral Positions Virus-Host Interactions and Structures The laboratories of Drs. L. Wayne Schultz and Tim Umland are seeking highly motivated individuals to participate in a joint interdisciplinary research project investigating Influenza A and

[ccp4bb] Chainsaw question

Hi all, I attempt to create a suitable model for molecular replacement using CHAINSAW. Whenever the program needs to mutate a Proline into something other than alanine I get "segmentation fault". This happens if the order of atoms in the proline residue is (N,CD,CA,CB,CG,C,O). Could you suggest

[ccp4bb] postdoc position in Greifswald, Germany

Dear All, In the group of Winfried Hinrichs a postdoctoral position/staff scientist is available for initially 3 years. The position involves research and teaching. Knowledge in German is not mandatory but might be helpful. You may test your skills with the official posting of the university r

[ccp4bb] postdoc position at Genzyme Corp

Dear all, I want to bring your attention to a crystallographer postdoc opening at Genzyme Corporation. Please see the job description below and for information on how to apply. Thanks very much! Ronnie Post Doctoral Associate Genzyme Corporation, ranked as one of the foremost biotechnol

Re: [ccp4bb] {Spam?} quick purification?

Mark, I agree with Artem in that you may find significant differences in the purity of eluants from the 'high fidelity' IMAC resins (I use pre-packed HisTrap columns). You may also want to have a look at the following reference for a comparison of a variety of different affinity tags in te

[ccp4bb] Postdoc in PX at SGC (U of Toronto)

I am recruiting for a postdoc position in my group, available immediately. This is the Protein Crystallography group of the Structural Genomics Consortium, Toronto. This position is in collaboration with The Chromatin Biology and Epigenetics Group. The remit of the Structural Genomics Consortium

[ccp4bb] Crystallization Reagent

Does anyone know of a source of AMP-N-PP? Thanks, --Paul

[ccp4bb] AW: [ccp4bb] Crystallization Reagent

Hi Paul, Check out http://www.jenabioscience.com/ Best Clemens > -Ursprüngliche Nachricht- > Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von > Paul Smith > Gesendet: Tuesday, August 11, 2009 7:46 PM > An: CCP4BB@JISCMAIL.AC.UK > Betreff: [ccp4bb] Crystallization R

[ccp4bb] model bias

Hi all, I am working with a protein that have 28% similar to my MR template. I have processed data in HKL2000 for one of my crystal and I got unique sol in space group P212121. with LLG 131 and TFZ score 13.5 I have used buccaneer and coot for model building and my Rfee came to 45%. I u

[ccp4bb] a simple question: how is redundancy measured?

For a given reflection (h,k,l) how much does each situation increase the redundancy ? and which maximizes the ability to measure anomalous differences? (so assume we're separating friedel pairs here) a) measuring the same reflection again b) measuring a symmetry related reflection c) both

Re: [ccp4bb] model bias

Great that you have MR phases - it will help you identify heavy-atom sites for phasing and perhaps even the sulphur sites will be enough. Poul On 11/08/2009, at 20.33, ManojSaxena wrote: Hi all, I am working with a protein that have 28% similar to my MR template. I have processed data in HKL