I guess someone has answered this?
Q1 Before detwinning data you must process in the lower symmetry..
Comment.
It may not bre necessary or sensible to use detwin - the resukltant
estimates of I are pretty unreliable..
You can use twinned data usually to solve an MR problem, and the
refine
I CANT BE SURE of what has happened BUT maprot is a very complicated
procedure and your script is trying to do 2 things at once - change the
cell and apply the mask.
I would try changing the cell first, then generating the mask, then
applying it
or else: generate mask, apply mask, then change
Can you send the command script?
Eleanor
kumar wrote:
Dear CCP4¹ers, I¹m trying to run DM for after Refmac on an MR solution but
it crashes. I do have the FOM¹s listed in my log file. I get a similar error
when I use the mtz file from Phaser¹s SAD + MR. I¹d appreciate any help.
Thanks! Kumar
A
I cant answer for cphasematch but I believe it uses a resolution
dependent phase analysis discussed by Woolfson and Lunin in the 1980s
and similar to the method used to calculate the MAPCC used in SHELXE
That checks for a possible origin shift as well as analysing
difference - but I presume
Dont forget the confusing interaction between recorded B factors in a
PDB file and TLS parameters. This Q is still I think unresolved but you
need to be careful!
Eleanor
Edward A. Berry wrote:
Interesting that the correlation between B-factor and
resolution didn't show up in the QDB as recentl
hi
I installed CCP4 with Coot downloaded as a binary from the CCP4 website on a
64bit Ubuntu 9.04.
The installation was flawless and the setup scripts ran like a charm.
however, when i type ccp4i to get the GUI.
i get the following error message:
exec: 4: /usr/local/xtal/tcltk++/bin/bltwish:
maybe those loops are right - ish.
Eleanor
kumar wrote:
Dear members,
I am refining a structure and I notice that when I delete loops that have
none, or very little density, the Rfree (which is around 40% at 3.4A) goes
up instead of going down. Much of the remainder of the molecule is in good
d
Hi all,
I have a few datasets that I think are twinned for several reasons.
1)The Rfree and Rmerge are both stuck very high.
2) The L-test for twinning in Scala shows twinning
3) Also the centric and acentric movements suggest twinning.
4) The spots do not match the predication from Mosflm (No
> -Original Message-
> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
> Rana Refaey
> Sent: Monday, August 03, 2009 12:26 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Twinning
>
> Hi all,
> I have a few datasets that I think are twinned for several reasons.
> 1)
Hello ccp4'ers
Where is / how can I obtain the resolution shell for each resolution
bin in scala? My eyes can't seem to find it.
Thanks
FR
-
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys
Thank you Eleanor and Kevin,
Eleanor suggested separating the two tasks. Eventually, I changed the cell
with maprot and then used MAPMAN to zero the map below the cut-off level.
Kevin described how maprot works and suggested down-scaling the map to have
more reasonable values for densities.
Thes
Hi all,
I am now preparing to label my protein with selenium-methionine for phasing. As
I have heard that selenium-methionine is prone to oxidation, so is it a must to
add reducing agent all along the purification? if so, which reducing agent and
what concentration is better ? and any alternati
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