Re: [ccp4bb] ctruncate problems

2009-07-08 Thread Stein, ND (Norman)
Dear Justin I have been unable to reproduce either of these problems. Perhaps something went wrong during the self compilation process? Something odd seems to have gone on even in the logfiles you classify as good. For example, in http://dev.gentooexperimental.org/~jlec/paste/good.log.html there

Re: [ccp4bb] ctruncate problems

2009-07-08 Thread Justin Lecher
Stein, ND (Norman) schrieb: > Dear Justin > > I have been unable to reproduce either of these problems. Perhaps > something went wrong during the self compilation process? Something odd > seems to have gone on even in the logfiles you classify as good. For > example, in > Thanks Norman, for the

Re: [ccp4bb] ctruncate problems

2009-07-08 Thread Justin Lecher
Stein, ND (Norman) schrieb: > Dear Justin > > I have been unable to reproduce either of these problems. Perhaps > something went wrong during the self compilation process? Something odd > seems to have gone on even in the logfiles you classify as good. For > example, in > > http://dev.gentooexper

Re: [ccp4bb] RMSD value is different from O and superpose

2009-07-08 Thread Eleanor Dodson
Are the chains numbered differently? the SUPERPOSE LSQ program requires you to specify which residues to superpose. And any RMS is wildly distorted by a few outliers.. Look at the list of > average differences to see if you have such a problem. The SSM superposition excludes outliers automatic

Re: [ccp4bb] Why NCS doesn't help?

2009-07-08 Thread Eleanor Dodson
There are many reasons why this could have ocurred. One is that your NCS operator is very close to being a crystallographic one. But you need to give more details of the problem before I can comment sensibly Eleanor Sun Tang wrote: Dear All, In refining my structure (two molecules in au) t

Re: [ccp4bb] Why NCS doesn't help?

2009-07-08 Thread Sun Tang
Dear Eleanor, Thank you very much for your reply. I think that the NCS operator is very close to the crystallographic two fold symmetry because when I searched for the solution with PHASER with one molecule as the model I could not find the solution so I have to use two molecules as the search

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-08 Thread Marius Schmidt
Yes, our new Akta prime has also only 2 mm path length and readings are, therefore, much smaller than with 1 cm path length in a typical spectro-photometer. Best Marius > Hi all, > > We recently got low UV reading on our AKTA prime. We contacted GE > healthcare, but was told that the UV reading