Hello all,
I'm just finishing up my first structure, a membrane protein with 5
fold NCS. The native dataset is good to about 2.1A. The R factors are good,
around .22/.26 without waters. The only problem is that I'm having a
difficult time fitting what looks to be a very important (potential
Hello all
I've got a situation where I have a P321 space group to about 8.0A, it
was able to scale and merge fine in hkl2000 but now I'm getting
negative scales for Scala. Any suggestions on low resolution scaling
and merging?
Thanks
FR
-
Franc
Hi All,
We recently upgraded to ccp4suite 6.1.1 version and have been
experiencing some issues while running refmac_5.5.0088 (for tls
refinement) through this ccp4 interface. We use these softwares
through sbgrid. I would really appreciate any help to troubleshoot
this issue.
The issue
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Hi Drew,
Have you tried arp/wARP (the LOOPY option) or the AutoBuild option in Phenix
? If you haven't tried this you can try a complete rebuilding using you
model as it stands and providing the complete sequence of your protein.
At this resolution (2.1A) , they may be able to rescue your loops at
Dear Drew,
Your loop region might or might not obey the ncs of the rest of the
molecule, so you should be sure to examine both the ncs averaged map
and each individual, nonaveraged, map.
You should be willing to accept, however, that this region may not
structured and it may not be possible
Hi Jerry,
did you check out the CCP4 wiki - there's a lot of information at
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Crystals
HTH,
Kay
Jerry McCully schrieb:
Dear All:
Recently we got some crystals from ammonium sulfate and PEG400.
They look like hexagonal single
Thanks for these suggestions. The protein is purified and crystallized in
the presence of substrate at all times. A reason that I'd like to assign all
the polypeptide density is that the remaining density in the active site
could be my substrate. I am fairly certain that the loops do not obey the
N
Dear All:
Recently we got some crystals from ammonium sulfate and PEG400. They look
like hexagonal single crystals but without sharp boundary. They did not
diffract well.
This protein can also be crystallized from PEG6000 in star shape. We tried
hampton additive screen but can no
Hello Annie,
How effective have you found using sparse matrix screens as
additives vs. traditional additive screens? I've tried this only a few
times without success and would like to hear from someone with more
experience.
Thanks!
Ho
UC Berkeley
--
Hi Ho--
We have had lots of success using the Hampton Research Crystal Screens 1 &
2 as additives. Sometimes these improve the crystal quality whereas the
96 Additive Screen does not. Whenever I screen for additives, I include
Crystal Screen 1 & 2. What I have NOT had a great deal of succes
The issue is:
While running tls and restrained refinement, the program doesn't perform
the given no. of tls refinements. For e.g., if you ask it to perform 10
TLS and 5 restrained refinement rounds, it'll run may be 2 or 3 rounds of
TLS and then jump to restrained refinement rounds and finish
Hello, everyone
I have a full-text ready to check. The editor asked me to specify the
separation distance between the stereo pairs, because my article contains
stereo-isomer images.
I made the stereo images with PyMol, but I really do not know the
"separation distance" used in the publication.
Can
hello all
i have cloned few proteins into PQE-30 vector,it express well into M15 host but
all of it goes into inclusion body,i have tried lower IPTG concn upto
.01mM,lower temp 16,25 as well but couldnt get my protein into soluble
fraction.can someone have any experience with this vector?
tha
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