Re: [ccp4bb] how to optimize crystal without sharp boundary

[Kay Diederichs]

Hi Jerry,

did you check out the CCP4 wiki - there's a lot of information at http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Crystals

HTH,

Kay

Jerry McCully schrieb:

Dear All:





     Can anyone give some suggestions to optimize such crystals?


     Thanks a lot,

Jerry

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Recently we got some crystals from ammonium sulfate and PEG400. They look like hexagonal single crystals but without sharp boundary. They did not diffract well. This protein can also be crystallized from PEG6000 in star shape. We tried hampton additive screen but can not turn them into single crystals. Hotmail® goes with you. Get it on your BlackBerry or iPhone. <http://windowslive.com/Tutorial/Hotmail/Mobile?ocid=TXT_TAGLM_WL_HM_Tutorial_Mobile1_052009>

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