It's also easier to fish the crystals out of the solution with a
hanging drop.
Simon
On 1 May 2009, at 06:35, Debajyoti Dutta wrote:
Hi,
>From the experiance of mine I can tell you that the crystal size
sometimes matters between these two methods. Hanging drop may yield
bigger crysta
Sorry, disagree again: with the right plate type (e.g. SwissCi plates),
it's far far easier from sitting drop, because:
1. you don't have to muck around with flipping over the cover slip,
instead just cut the seal
2. you have more time, because your drop does not evaporate as quickly
(see earli
Dear Herman.Schreuder,
Perhaps there is some misunderstanding about my question. There is
one molecule in the asymmetric unit (showed as symbos A). After the 2nd
crystallographic symmetric operation, another molecule appears (symbol B).
However, the density show that there is one
A trick that small molecule crystallographers use is to make a packing
diagram where the volume includes both molecules A and B, or maybe even
two of each. In this case, it would display all of C (or maybe even 2 of
C either twisted back on itself (symmetry element near molecule C) or
extendin
Further to the other contributions to this discussion, please
note that the Oxford Diffraction PX Scanner system - for diffraction
quality assessment of crystals in situ in the crystallisation plate,
can also play a powerful role in all of this. Thus, in addition to
differentiating salt from p
Sure, there are differences between these two methods, but no systematic
study has been reported showing one is better than the other in terms of
getting initial hits. Since we have a crystallization robot, I routinely
set up sitting drops for initial screens and hanging drops (manually)
for op
My home institution, in effort to cut costs, is making an effort to push those
of us on Macs onto PCs. Up till now they have been very generous via a lease
program for computer hardware, but that is changing given the current
economics. The institution currently does not support Linux so we ar
Title: Computer hardware and OS "survey"
Well, Coot, O, Pymol, CNS, and CCP4i, as well as
Open-EPMR all have Windows versions. The main issues with a Windows
workflow are (1) jobs will run significantly slower than in Linux, and
(2) the DOS command shell is not as powerful as Linux, although it
If you have found Bill Scott's pages on Macs useful, you may find his
Ubuntu pages similarly useful: another reason to go with linux over
windows. There are also people creating rpm packages to easily install
the likes of coot on Fedora/RHEL/Centos, another well supported option.
Also, phenix,
On Friday 01 May 2009, Link,Todd M wrote:
> My home institution, in effort to cut costs, is making an effort to push
> those of us on Macs onto PCs. Up till now they have been very generous via a
> lease program for computer hardware, but that is changing given the current
> economics. The ins
Hi
Most of the software for macromolecular crystallography data analysis will
run happily on Linux, OS X or Windows. However, Windows is much more
GUI-based and much less open to shell scripting than UNIX based systems
like Linux or OS X (DOS-Shell is very inflexible, even compared to sh or
csh, l
I have found that cygwin works quite well for doing unix-type things on
windows--one can use whatever shell one likes, run perl scripts, etc. Have
people had problems with cygwin? I used to run CNS on it all the time.
Jacob
***
Jacob Pearson Keller
North
Hi Todd:
One option on Windows is to install Ubuntu in a mode that lets it run
nested as a guest in a window within the host operating system. This
is now one of the options on the (free) Ubuntu install CD. I've
actually not tried it, so I can't tell you how good it is, but my
guess is
Cygwin has problems with Vista.. you need to run it as administrator.. works
fine with XP though. Moreover, Cygwin is not very well supported anymore, as
the developer works for Red Hat now. If it is just the X, there are other
free programs.. XMing is quite good.
You can indeed install Ubuntu und
I used to have a Mosflm executable running under Cygwin. It ran just like
it does on other platforms (as far as I can recall). So did the CCP4 I
built. But the bash it (Cygwin) came with seemed to be incomplete, and the
tcsh (that I prefer to use) also didn't seem to be implemented terribly
well. T
> no systematic study has been reported
We had a ACA poster many years ago, comparing same drops of various
proteins in linbro plates, 98 conditions hanging and sitting drop,
on sitting posts, and with added glass shards (attached gif).
In summary, comparable overall success rate, but variation
Todd,
Once upon a time I studied at an institution of higher learning. Its specialty
is (and was) the education of and participation in medical sciences (I guess
that?could be?an oxymoron, sorry). With that comes the securely keeping and
sharing (as needed) of patient data. The institutional bu
Hi Todd,
I've found, in two institutions I have now worked for, that the
tradeoff is between IT support and the hardware/software you want to
use. When our group moved institutions recently our new IT department
told us that they would only support managed desktops on windows
machines. We
Link,Todd M wrote:
... I did not find an equal web support page for Windows.
It just isn't needed. If there is a windows version of a program you get
to "download, install, run" and then get on with your life.
Jon
My advice:
Embrace virtualization for all tasks except interactive 3D visualization.
If you're not yet familiar with VMware, Parallels, or open-source work-alikes,
then it is high time you joined the revolution --- the rest of us have been
running Mac OS X, Linux, and Windows on the same ha
Not to surprisingly much of the response had been go linux. I was trying to
head off that advice with the "university doesn't support Linux". And if I
was more of a real man (but sadly it is not so, but I try to wear my girly-man
credentials with as much pride as I can muster) I would go com
I'm surprised that dual booting hasn't been brought up yet. A dual boot
machine has two (or more if you like) operating systems installed to
different hard drive partitions, and switching between them is as simple
as restarting. All major distributions of linux nowadays come with the
option t
As Warren pointed out, dual-boot is so 20th century it's surprising people
still bother with it. For me, dual boot (never mind it was on a fantastic
Thinkpad) was the major reason to go for OSX. I was simply too sick of
it.. It might sound like heresy to true Macolytes but I feel I have now the
Hello fellow members of the forum,
Protein expression related question.
We need some recomendations for a reliable and quality
service for production of bacculovirus pellet in bulk.
people can send private email me.
Thanks for the attention.
Padayatti PS
--
Pius S Padayatti
Phone: 216-658-4528
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