[ccp4bb] Workshop "Diffraction Data Collection Using Synchrotron Radiation" (fwd)

After the successful first workshop on "Diffraction Data Collection Using Synchrotron Radiation", which took place in 2007, a second edition of the workshop will be held from August 13-15, 2009 at the Helmholtz Zentrum Berlin fuer Materi

[ccp4bb] first year of CCP4 user wiki, and XDS wiki - some statistics

Dear all, I missed the first anniversary of the CCP4 user ( http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Main_Page ) and XDS ( http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Main_Page ) wikis on Feb 7! Nevertheless it appears useful to look back: Month Un

Re: [ccp4bb] Problems with phasing a protein (1300aa)

i was just refering to the mentioned "ideal case" with 43 se and 1300 aa in AU, and some real (successful) examples of same size range. -tommi Quoting "Bernhard Rupp" : What is not true? Also in your case the applet estimates an expected 4-5% signal which is quite doable with decent data. B

Re: [ccp4bb] images

Kay Diederichs wrote: In this case the structure factors were deposited, but these do not have a column for the anomalous signal. Re-refinement with these structure factors was inconclusive. If I could have downloaded the images, I could have investigated this easily, because there's a large

[ccp4bb] Confusing Diff Map peaks

  Hi All, I am refining one 1.9A structure with refmac5. I just found that there are some difference map peaks developed but those positions are not shared by the 2Fo-Fc map beyond 1.3 SIGMA level. The shape of the difference map looks like the same as the nearest electron density map. This i

Re: [ccp4bb] images

Now that there is possibility that images could be fake, which means the first and automatic FFT in crystallography could be falsified. All those depositions later than this would be just nothing but wrong, if images were fake. To keep the first and most important FFT not contaminated, I would su

Re: [ccp4bb] images

Hi, Sorry for the long post - this stirred some memories. Feel free not to read below this line :) --this line-- The suggestion to store frozen crystals brings to mind an old (and bad!) Soviet science fiction novel (The Seller of Air) - only instead of storing liquified oxygen, we're looking at

Re: [ccp4bb] Problems with phasing a protein (1300aa)

Kumar wrote: Hello CCP4bb members, I have been trying to obtain phases for a protein which contain ~1300aa. We have obtained native data to a resolution of 3.3A (Space group I222 or I212121). But we are having tough time phasing it. 'Se' labeled crystals diffracts maximally up to 3.5 to 4 A