Hi All, I am refining one 1.9A structure with refmac5. I just found that there are some difference map peaks developed but those positions are not shared by the 2Fo-Fc map beyond 1.3 SIGMA level. The shape of the difference map looks like the same as the nearest electron density map. This is surface region, though there B factors are not high (around 25).
Thank you for your suggestions and help. Sincerely Debajyoti Dutta
