Dear all,
sorry for the off-topic and possibly very naive question- but does anyone
know what happens if normal protein is put in detergent-containing aqueous
solution? how much detergent can a regular protein tolerate? I was trying to
search literature but couldn't find any...
Thank you greatly
Helps sometimes, Start with:
A. McPherson, S. Koszelak, H. Axelrod, J. Day, R. Williams, L. Robinson,
M. McGrath and D. Cascio (1986) An experiment regarding crystallization
of soluble proteins in the presence of beta-octyl glucoside. J Biol Chem
261:1969-1975.
Twenty-one soluble proteins, five t
Melody,
While Joe addressed the possible effects of detergents on the
crystallization of proteins, the possible effects (both negative and
positive) of detergents on proteins are quite variable. Often there
are no general rules except to avoid ionic detergents (from anionic
detergents l
Dear community,Dear community,
This is an announcement for a EMBO/MAX-INF2 practical course
X-ray crystal structure determination of macromolecules
to be held at the Soleil Synchrotron near Paris (France), from the 14th-21st of
September 2008.
This course is mean
Hi ccp4'ers:
Thanks for the quick response on the coot side chain trimming issue.
---
I received an email from Patricia Legler to try Chainsaw (a ccp4 program I'm
not familiar with) and clustal to trim sidechains.
Paul Emsley tweaked my scheme script which I retweak and present here .
Paul
Hello,
A more precise view on the spectroscopic signature of your crystalline
heme protein can be obtained by microspectrophotometry (UV-vis
absorption, Raman or fluorescence). For such studies, people are
welcome to apply for using the Cryobench lab at ESRF
(http://www.esrf.eu/UsersAndScien
Dear BB,
I have a structure of a protein domain that binds to a peptide ligand
containing either a lysine or an arginine. There are homologs in the PDB with
the peptide in complex both with lysine and arginine.
I would like to model both alternative ligands bound to the new domain.
It looks lik
Hi I am trying to exclude a bad wedge of data during scaling in scala in
the newest ccp4
( fink install this morning from W.G Scotts sage.ucsc Binaries ..so they
should be version 6)
The batches I need are
1 to 200 and 400-720
I have clicked the "Override automatic definition of runs to mark
disco
Hi.
Based on your explanation it looks like you want to remove your "RUN 1
REFERENCE" and "EXCLUDE BATCH 400 TO 500" lines from your script. You might
also want to try without the NAME lines on the off chance that the harvesting
stuff is causing the problems.
My best guess is that the "RUN 1
In ccp4i Scala task, click to open the "Excluded data" panel, click on
"Exclude selected batches"
There you can define one or more ranges of batches or lists to exclude
If you just want to exclude the last part you can define a range eg
301 to 999
You don't need to explicitly define runs
Hi.
I did try that beforehand when I tried excluding a "range" of batches with
the ccp4i gui
But I got an error
Scala: *** Gap in "time" (rotation) ***
Sorry...both versions of the protocol for handling a bad internal wedge are
giving me either a "gap in rotation" error or a "Run 2 has no
Dear all,
Sorry for this off-topic question.
I am studying a peripheral membrane protein (13kDa) by liquid state
NMR, which is stable at pH 9.0 (50mM CHES pH9.0, 150mM NaCl and 1mM
DTT). It crashes when I lower pH of buffer. The addition of 50mM Arg +
Glu works to stabilize the protein at low pH,
12 matches
Mail list logo
