Helps sometimes, Start with: A. McPherson, S. Koszelak, H. Axelrod, J. Day, R. Williams, L. Robinson, M. McGrath and D. Cascio (1986) An experiment regarding crystallization of soluble proteins in the presence of beta-octyl glucoside. J Biol Chem 261:1969-1975.
Twenty-one soluble proteins, five tRNAs, and three protein-nucleic acid complexes were studied in a systematic manner with regard to their crystallization behavior from polyethylene glycol and ammonium sulfate solutions in the presence of 0 to 1.5% beta-octyl glucoside. Our observations suggest that this neutral detergent does influence in a very positive way the growth characteristics of the macromolecules included in this experiment. In general, more reproducible and rapid growth was noted with an increased number of large individual crystals at the expense of microcrystals. In several cases, new crystal forms were discovered. Selected x-ray diffraction analyses imply that crystals grown in the presence of beta-octyl glucoside diffract as well or better than those grown in its absence. In addition, a screen of two proteins grown in the presence of 14 different common detergents suggested that a general detergent effect may be beneficial for the growth of crystals of biological macromolecules. Then check out the detergent screens from Hampton and others Joe Becker - Merck Research Labs ________________________________ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Melody Lin Sent: Friday, March 14, 2008 9:40 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] normal protein in detergent? Dear all, sorry for the off-topic and possibly very naive question- but does anyone know what happens if normal protein is put in detergent-containing aqueous solution? how much detergent can a regular protein tolerate? I was trying to search literature but couldn't find any... Thank you greatly for your attention and inputs. Best, Melody ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------
