Dear everyone,
sorry for the non-CCP4 question.
I would to process the image files (.sfrm file) from Bruker X8 proteum by
HKL2000. Is there anybody have a def.site file of HKL2000 that works with
the .sfrm images taken by the Bruker's Proteum Pt135 CCD detector?
Thanks in advance!
Best Regard
Dear Shi,
The def.site file is specific for each individual CCD detector, so you
cannot use any other def.site file from any other user of an X8Proteum
system.
If you do, indexing and hence processing will not work as it should.
If you have a HKL2000 license, there should be a def.site present
Dear ccp4 comrades,
I have a problem I hope someone can help with. I am attempting to
index and integrate
a number of datasets which will all index (with mosflm) and scale
(with scala) in P21 with
low penalty scores and low Rmerge respectively.
However, when ipmosflm integrates, about 75% o
Kendall, All,
One classic example that comes to mind is the multiple binding modes
that have been structurally described for the drug Gleevec AKA Imatinib.
Gleevec or closely related analogs have been shown to bind to the
kinases Abl (1) and c-Kit (2) in a Phe-out conformation of the conserved
Hi Kendall,
in cAMP-dependent protein kinase (PKA) in complex with the bisindolyl maleimide
inhibitor BIM-2, two conformations of the inhibitor are seen in the two kinase
molecules of the asymmetric unit.
Gassel M, Breitenlechner CB, König N, Huber R, Engh RA, Bossemeyer D.
The protein kinase C
Andrew Dore wrote:
Dear ccp4 comrades,
I have a problem I hope someone can help with. I am attempting to
index and integrate
a number of datasets which will all index (with mosflm) and scale
(with scala) in P21 with
low penalty scores and low Rmerge respectively.
However, when ipmosflm integrat
Kendall,
The story has been published. The lead paper is here:
Norman, B. H.; Dodge, J. A.; Richardson, T. I.; Borromeo, P. S.; Lugar, C.
W.; Jones, S. A.; Chen, K.; Wang, Y.; Durst, G. L.; Barr, R. J.;
Montrose-Rafizadeh, C.; Osborne, H. E.; Amos, R. M.; Guo, S.; Boodhoo, A.;
Krishnan, V.;
If the number ~75% was ALWAYS true for all datasets, while not a SG error,
not cracks/twinning, not a mosaicity problem and not here and there, then
I would BET the REAL oscillation angle for each img for all datasets
should be 4 times as what what input while the steps stayed correct. (For
instanc
All;
Has anyone been able to compile Tcl/Tk and Blt for Mac
OS X Leopard (10.5.2)? I'm having problems with the
out of the box distribution. When I run the
build_tcl_tk.sh script, it stops with the following
error:
.
.
.
Dear Steven,
I've struggled with this for the longest time. Neither the source from
ccp4 nor from sourceforge works. What did the trick for me was to
download the entire ccp4 distribution
ftp://ftp.ccp4.ac.uk/ccp4/6.0.2/binaries/ccp4-6.0.2-osx-i386.dmg.gz
This contains a package for tcl/tk an
Apologies for the duplicate message, it seems the link in the previous
message might now work.
Here is it again, corrected.
__
From: Remacle, F (Francois)
Sent: 19 February 2008 16:06
To: CCP4-dev; '[EMAIL PROTECTED]'
Subject:CCP4 web-
Dear users and developers,
This is my great pleasure to announce the release of the long awaited
winter 2007-2008 issue of the web-based CCP4 newsletter.
I hope all of you will enjoy the reading of these interesting articles.
You can find the newsletter and the past issues at
www.ccp4.ac.uk/n
Hi,
Agreed on this. I used to have issues with aggregation due to the Nickel
being stripped off the column (happens with elution in imidazole). Adding
10mM EDTA to the elution immediately AFTER it has come out of the column
will chelate the Ni++ and prevents aggregation (at least in my case).
Afte
I've been fighting a similar problem here on linux. My solution
was to "downgrade" the tcl + blt packages to use tcl version 8.4.
Tcl version 8.5 is not working happily here with ccp4i.
That still leaves me with a few CCP4 components that complain
about version incompatibilities, but I assume tho
An added benefit of EDTA is that it inhibits some proteases - for one
of our wimpier proteins, spiking each fraction collector tube with a
little EDTA before running the Ni column really helped reduce keep
the sample in one piece.
Phoebe
At 01:18 PM 2/19/2008, Sophia Tsai wrote:
Hi,
Andreas;
Thanks for the suggestion. The distribution you
pointed me at
doesn't work either, the bltwish application
segmentation faults.
It might be a 64bit vs 32 bit thing, since I'm using a
Mac Pro 64 bit
machine. I guess I get to go back to the makefile for
blt.
Thanks again,
Steve
--- An
Hi Ethan;
I got the distributed version to work -- you can't use
the
installer to put the program in anything other than
the /usr/local/
filesystem.
It turns out I can't use fink effectively since I need
two things: To
install from behind a firewall (a pain with fink) and
portability of executa
Dear all,
Where is a good place to order brom-cytosine or iodo-dC? Thanks.
Best,
Jinjin Zhang
Hi Jacob,
Two points which I don't think others have mentioned:
Have you checked the pH of your imidazole (or pHed the solution before
elution)? It may be that the imidazole has driven up your pH, causing
protein to crash out.
You can add various things to fraction tubes prior to elution so t
Sorry, I just figured out how to post to the CCP4bb instead of sending a
private email.
Here are two missed messages that I think should appear on the board:
- Original Message -
From: "Miles Pufall" <[EMAIL PROTECTED]>
To: "Zhijie LI" <[EMAIL PROTECTED]>
Sent: Tuesday, February 19, 2
A postdoctoral position is available in the laboratory of Macromolecular
Structure, Institute of Molecular and Cell Biology, Singapore.
The laboratory is fully equipped for biochemical experiments, and with
state-of-the-art crystallographic facilities including a Rigaku
FRE X-ray generator eq
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