If the number ~75% was ALWAYS true for all datasets, while not a SG error, not cracks/twinning, not a mosaicity problem and not here and there, then I would BET the REAL oscillation angle for each img for all datasets should be 4 times as what what input while the steps stayed correct. (For instance, data might be collected in the way like 0-4, 1-5, 2-6,...while ipmosflm took as 0-1,1-2,2-3...) Please also check you high resolution date with contrast enhanced in thoese region.
LJL > Dear ccp4 comrades, > > I have a problem I hope someone can help with. I am attempting to > index and integrate > a number of datasets which will all index (with mosflm) and scale > (with scala) in P21 with > low penalty scores and low Rmerge respectively. > > However, when ipmosflm integrates, about 75% of the spots below 7 > angstroms are > not predicted and even some spots to 4 angstroms are not predicted. > > Changing to higher or lower symmetry spacegroups does not help, > increasing mosaicity > helps to increase marginally the number of spots being integrated, > but I don't think > this is the problem. Xtriage etc. show the data is not twinned. > > Any help/ideas would be very welcome ..... > > Cheers, > > Andy Dore > > The Institute of Cancer Research: Royal Cancer Hospital, a charitable > Company Limited by Guarantee, Registered in England under Company No. > 534147 with its Registered Office at 123 Old Brompton Road, London SW7 > 3RP. > > This e-mail message is confidential and for use by the addressee only. If > the message is received by anyone other than the addressee, please return > the message to the sender by replying to it and then delete the message > from your computer and network. >
