[ccp4bb] refmac5: FIND_CONTACT_WITH_BRICKING failed

-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Good morning, I compiled the latest Version of refmac5 (5.3.0040) and installed the latest library. When I want to read in a glycerol with coot and also when running buccaneer, refmac bails out with the above error message Too many symme

Re: [ccp4bb] tricky molecular replacement

Yes- ALMN seems to have given one excellent match between the data sets which is encouraging. But since you are matching hexagonal to trigonal you get a large number of symmetry equivalent peaks, relating a molecule from the P1 set to one of the hexagonal set. The angles are defined so that: "T

Re: [ccp4bb] tricky molecular replacement

I would stop trying to use a map! that is an act of desperation when you have uninterpretable but possibly somewhat true experimentally phased density.. If you have a model it is MUCH MUCH easier! So as I said - I would find the best hexagonal dimer - you may know this or you can dispatch the s

Re: [ccp4bb] protein degradation?

Hi, I also had a similar problem with one of my proteins... I had it cloned in two different plasmids, one with Cter His-tag and the other in the Nter. Whenever I purified it using IMAC purification I would get the double band (that I confirmed by MS and were the same). I got ride of this "double

Re: [ccp4bb] protein degradation?

Hi, if I recall this correctly, it is the nickel that is in your sample after elution and boiling your protein in SDS sample buffer does the rest. So, could be the sample is fully okay!!! J. Tiago Botelho wrote: > Hi, > > I also had a similar problem with one of my proteins... I had it clon

[ccp4bb] CCP4i working with PC cluster system

Dear ccp4bb, I am in trouble to execute ccp4 jobs on CCP4i with a PC cluster system. Sun Grid Engine, we are using, the job management application running on the Linux cluster, sometimes has troubles to synchronize with CCP4i well. 0. Batch job setting is modified to be suitable for our system

[ccp4bb] post a message

I would like to post a message in CCP4 bulletin board. A Postdoctoral Researcher position for structure determination of a membrane protein complex is open at Paul Scherrer Institut, Villigen Switzerland. The candidate should hold a PhD in biological sciences and have

Re: [ccp4bb] protein degradation?

Let me correct myself, it is the imidazole buffer (not the nickel) and boiling your protein in does the rest I will try to find the exact reference for this phenomenon. J. Jeroen Mesters wrote: Hi, if I recall this correctly, it is the nickel that is in your sample after elution and boiling

[ccp4bb] Apple OS 10.5 and X11

After Bill Scott's and Juergen Bosch's comments about upgrading to Leopard 10.5, it has been quiet. As I am upgrading some "non- essential" machines up to 10.5, I just wanted to check if there is more info out there. Despite horror stories on the discussion site, upgrading 1 G4 PB, an Int

[ccp4bb] libccp4map.so

Does anyone know where I can find a copy of "libccp4map.so" for Mapslicer please ? Thanks, Iain

Re: [ccp4bb] libccp4map.so

Sorry, to clarify the library is missing from my system (wasn't compiled during CCP4 installation)..I need a copy to add to $CLIB. Thanks, Iain Iain Kerr wrote: Does anyone know where I can find a copy of "libccp4map.so" for Mapslicer please ? Thanks, Iain