Thanks a lot for the replies. The option which allows to set the chirality
restraints to "both" was exactly what i was looking for, since i use refmac for
refinement. Surprisingly, the binding site seems not to be stereoselective in
this case.
greetings, david
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You can use any acronym and your dictionary file overrides any existing
entry.
but check at the RSCB or EBI if your ligand already has a defined ID.
Isnt a dsn6 file a map file, whereas an mtz file is a list of reflections?
You can calculate a CCP4 map from your mtz file, then read that into O
I have a GRASP session saved which I startup by reading in "grasp.state". I
have changed some colours in "defcol.dat" and I would like these changes to
take effect in my saved session, but everytime I read in "grasp.state" it
changes the colour palette back to the default set again.
Does anyone kn
Hi, Phoebe,
Sorry to jump in late on this one- but I second Stefan's note here. When
soaking dinucleotides (which are poor substrates) into Klenow Fragment xtals, I
noted binding both at the active site and at a crystal interface site that is
likely nonphysiological. The adventitious site is
Here's another late addition to the discussion, which Chad's comment
reminded me
of:
We just deposited a similar-sounding structure of a DNA polymerase
(2HVI). The
protein is in the closed conformation, with ddCTP pairing with G in the active
site of the two monomers in the ASU. A third ddCT
Hi,
Sorry to bother you all.I'm going to try to reproduce some results
from the initial screening from PACT kit.The conditions are something
like 0.1M PCB buffer, 25% w/v PEG 1500 and 0.1 MMT buffer, 25%w/v PEG
1500. I was just wondering is there any easy way to get these
buffers?Do you happen to
