Pascal,
The reason this phenomenon looks odd to you is that detergent phase separation
is not a micelle size phenomenon, but a micelle surface phenomenon. Like any
colloidal solution, even protein, there can be conditions where the colloidal
particles aggregate (the cloud point), creating a mi
Hi Pascal,
this behavior doesn't surprise me.
We had crystals in FC12, and didn't get any phase transitions in any
PEGs from 200 to 20.000, and with or w/o organic solvents such as MPD.
And I can tell you we had a lot of detergent with the purified protein,
concentrating on 50kDa.
With lipids,
Pascal,
Not personally observed this myself, but just wondering, as a control, have you
tried setting trays with just the buffer+detergent (no PDC)? And if so, do you
see the same behavior or not? Also, do you see phase separation in all drops
or just a majority? Do the drops that exhibit th
