Kendall is absolutely correct..
Faisal
On 3/30/11, Kendall Nettles wrote:
> There is a paper from John Kuriyan on co-expressing a phosphatase with c-Src
> kinase domain to enable bacterial expression of homogenous protein (
> PMID:
> 16260764). Also look at work from E. Goldsmith (PMID: 16829129
There is a paper from John Kuriyan on co-expressing a phosphatase with c-Src
kinase domain to enable bacterial expression of homogenous protein (
PMID:
16260764). Also look at work from E. Goldsmith (PMID: 16829129)
Lastly, I would suggest general approaches, such as: varying the ends of the
DNA
You can use ATP agarose for purification and include the cofactor required
right from expression till purification steps.
On Tue, Mar 29, 2011 at 8:10 PM, Neeraj Kapoor wrote:
> Hi All,
> I am trying to express a kinase but unfortunately there is aggregation
> happening as the protein is pur
