You can use ATP agarose for purification and include the cofactor required right from expression till purification steps.
On Tue, Mar 29, 2011 at 8:10 PM, Neeraj Kapoor <nkap...@mail.rockefeller.edu > wrote: > Hi All, > I am trying to express a kinase but unfortunately there is aggregation > happening as the protein is purified over a column. SInce I am new to the > field of kinase expression and purification, I was wondering if someone > could provide me with a couple of good references that can hit the ground > running for me. I would also very much appreciate any helpful suggestions > that anyone might have. > > thanks > Neeraj
